2001-03-28 07:42:47| 人氣1,143| 回應0 | 上一篇 | 下一篇

4th Try

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Condition: 白天變長了
Temperature: 25 C
Aim: try 3 different concentrations of agarose gel. 1.1% 1.8% 2.5%, consider with xylene cyanol loading buffer

Discussion:
1. 1.1 %, ran over, only 1000 bp band showed on the gel.try again with other loading buffer.
2. 1.8 %, good seperation between 400bp~600bp. similar result as 2nd try (II)
3. 2.5 %, the bigger pieces of DNA didn't run very far, but the small pieces, not very good gel this time, need to try again.

Conclusion:
DNA run slower as increase concentration of agarose gel.

台長: 爛藍懶人
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