2001-03-25 10:34:52| 人氣1,110| 回應0 | 上一篇 | 下一篇

3rd Run (I ) 修正

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Condition: 做惡夢的懶人
Temperature: 30 C
Aim: try high voltage 10v/cm, and high conc. of metaphor agarose gel 2.5 %

Discussion:
1. difficult to dissolve metahphor agarose gel when too concentrated.
2. gel ran even faster than last time. ~Bromophenol blue did run faster, but not for DNA pieces.
3. no much steam comes out, the temperautre is low.
4. bands are fade, difficult to see the seperation between. ~but bigger seperation between small DNA pieces.
5. less difficulty during staining, due to the stronger gel.

Concusion:
high concentration makes gel stronger, DNA ran slower, not good for resolution.



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